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Recent and Selected (*undergrad, **grad, ^co-first author)

Meiotically dividing spermatocytes
Inactive X chromosome (pink) in spermatocytes

Shakes DC. Sperm bud mitochondria to adjust the numbers. Nat Cell Biol. 2023 Nov;25(11):1564-1565.

Mei X, Maniates KC, Looper A, Krauchunas AR, Druzhinina M, Dharia S, Ni J, Singaravelu G, Gu S, Shakes DC, Grant BD, and AW Singson (2023) SPE-51, a sperm-secreted protein with an immunoglobulin-like domain, is required for fertilization in C. elegans. Current Biology,

Al-Yazeedi T, ^Xu EL*, Kaur J, Shakes DC, Pires-daSilva A (2022) Lagging X chromatids specify the orientation of asymmetric organelle partitioning in XX spermatocytes of Auanema rhodensis. Genetics 222(4). doi:10.1093/genetics/iyac159.

Ragle JM, Morrison KN*, Vo AA, Johnson ZE, Lopez JH, Rechtsteiner A, Shakes DC, and Ward JD (2022) NHR-23 and SPE-44 regulate distinct sets of genes during C. elegans spermatogenesis. G3 Genes/Genomes/Genetics.

Peterson, JJ*, Tocheny CE*, Prajapati G, LaMunyon CW, and DC Shakes (2021). Sub-cellular patterns of SPE-6 localization reveal unexpected complexities in C. elegans sperm activation and sperm function. G3 Genes/Genomes/Genetics jkab288 

Morrison, KN*; Uyehara, CM**; Ragle, J. Matthew; Ward, JD; Shakes, DC. (2021) MFP1/MSD-1 and MFP2 co-localize with MSP during C. elegans spermatogenesis. microPublication Biology.

Van Goor J, Shakes DC, and Haag E.S. (2021). Fisher vs. The Worms:  Extraordinary Sex Ratios in Nematodes and the Mechanisms that Produce Them. Cells 10:10.1793.  

Price KL**, ^Presler M*, Uyehara CM**, and DC Shakes (2021) The intrinsically disordered protein SPE-18 promotes localized assembly of MSP in C. elegans spermatocytes. Development: doi: 10.1242/dev.195875

Ragle JM, *Aita AL, *Morrison KN, Martinez-Menez R, Saeger HN, Ashley GA, Johnson LC, Schubert KA, Shakes DC, and JD Ward (2020) The conserved molting/circadian rhythm regulator NHR-23/NR1F1 serves as an essential co-regulator of C. elegans spermatogenesis. Development 147(2).

 Yin, R., Harvey, C., Shakes, D. C., Kerscher, O. (2019) Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins. J. Vis. Exp. (146), e59576, doi:10.3791/59576.

Tandonnet S., **Farrell M.C., Koutsovoulos G.D., Blaxter M.L., Parihar M, Sadler P.L., Shakes D.C. , Pires da Silva A. (2018) Sex- and gamete-specific patterns of X chromosome segregation in a three-gendered nematode. Current Biology 28 (1): 93-99.

**McCaig CM, *^Lin X, **Farrell M, *Rehain-Bell K, Shakes DC (2017) Germ cell cysts and simultaneous sperm and oocyte production in a hermaphroditic nematode. Dev Biol. 430(2):362-373. doi: 10.1016/j.ydbio.2017.08.010.

*Winter ES, Schwarz A, Fabig G, Feldman JL, Pires-daSilva A, Müller-Reichert T, Sadler PL, Shakes DC. (2017). Cytoskeletal variations in an asymmetric cell division support diversity in nematode sperm size and sex ratios. Development 144(18):3253-3263. doi: 10.1242/dev.153841.

Singaravelu G, Rahimi S, Krauchunas A, Rizvi A, Dharia S, Shakes D, Smith H, Golden A, Singson A. (2015) Forward Genetics Identifies a Requirement for the Izumo-like Immunoglobulin Superfamily spe-45 Gene in Caenorhabditis elegans Fertilization.Curr Biol. 25(24):3220-4.

Chu DS and Shakes DC. (2013) Spermatogenesis. In Germ Cell Development in C. elegans, Editor Tim Schedl. Springer Science.  Advances in Experimental Medicine and Biology. Volume 757: 171-203.

Kulkarni M, Shakes DC, *Guevel K, and Smith HE. (2012) SPE-44 implements sperm cell fate in Caenorhabditis elegans. PLoS Genetics 8(4): e1002678.

Shakes DC, Miller DM, and Nonet ML.  (2012) Immunofluorescence Microscopy. In Caenorhabditis elegans, Part B, 2nd edition, Methods in Cell Biology Volume 107: 35-66. Editor Joel Rothman and Andy Singson.

Geldziler B, Marcello MR, Shakes DC, and Singson A. (2011) The Genetics and Cell Biology of Fertilization. In Caenorhabditis elegans, Part A, 2nd edition, Methods in Cell Biology Volume 106. 343-75. Editors Joel Rothman and Andy Singson.

Shakes DC. (2011) For male Caenorhabditis elegans, sperm activation is a “just-in-time” event. (2011) PLoS Genetics 7(11): e1002392. doi:10.1371/journal.pgen.1002392.

Shakes DC, Allen AK, *Albert KM, Golden A (2011) emb-1 Encodes the APC16 Subunit of the Caenorhabditis elegans Anaphase-Promoting Complex. Genetics 189: 549-560.

Diane C. Shakes (corresponding author), *Bryan J. Neva, Henry Huynh, Jyotiska Chaudhuri, Andre Pires-daSilva.  (2011) Asymmetric spermatocyte division as a mechanism for controlling sex ratios.Nature Communications 2:157. DOI: 10:10.1038/ncomms1160.

Shakes DC, J Wu, P.L. **Sadler, K. LaPrade, L.L. Moore, *A. Noritake, D.S. Chu. (2009) Spermatogenesis-specific features of the C. elegans meiotic program. PLoS Genetics August 5(8): e1000611

Chatterjee I, **Richmond A, Putiri E, Shakes DC, and Singson A. (2005) The Caenorhabditis elegans spe-38 gene encodes a novel tetraspan integral membrane protein required for sperm function and fertilization. Development 132: 2795-2808.

Frazier T,  ^Shakes D, Hota U, and Boyd L (2004) Caenorhabditis elegans UBC-2 functions with the anaphase promoting complex but also has other activities. J. Cell Science 117: 5427-35

Shakes DC, ^Sadler PL, Schumacher JM, *Abdolrasulnia M, and Golden A. (2003) Developmental defects observed in hypomorphic anaphase-promoting complex mutants are linked to cell cycle abnormalities. Development 130: 1605-1620.

Davis ES, ^*Wille L, Chestnut B, Sadler P, Shakes D, and Golden A. (2002) Multiple subunits of the C. elegans Anaphase Promoting Complex are required for chromosome segregation during meiosis I. Genetics 160: 805-813.

Morton DG, Shakes DC, Nugent S, *Dichoso D, **Wang W, Golden A, and Kemphues KJ. (2002) The C. elegans par-5 gene encodes a 14-3-3 protein required for cellular asymmetry in the early embryo. Developmental Biology 241: 47-58.

Golden, A., ^**Sadler PL, Wallenfang MR, Schumacher JM, Hamill DR, *Bates G, Bowerman B, Seydoux G, and Shakes DC (2000). Metaphase to anaphase transition defective (mat) mutants in Caenorhabditis elegans.  J. Cell Biol. 151: 1469-1482.

**Sadler, P.L. and D.C. Shakes. (2000) Anucleate C. elegans sperm can crawl, fertilize oocytes, and direct anterior-posterior polarization of the 1-cell embryo. Development 127(2): 355-366. 

**Wang, W. and Shakes, D.C. (1996) Molecular evolution of the 14-3-3 gene family. J. Mol Evol. 43: 384-398.

Epstein, H.F. and Shakes, D.C., editors (1995). Caenorhabditis elegans: Modern biological analysis of an organism. Methods in Cell Biology Volume 48. Academic Press. San Diego.

Shakes, D.C.  (1995) Fostering creativity in the science classroom: A science and the arts project. J. Coll. Sci. Teaching 24: 333-335.

Shakes, DC and S Ward. (1989). Initiation of spermiogenesis in C. elegans: A pharmacological and genetic analysis. Dev. Biol. 134: 189-200.

Shakes, DC and S Ward (1989). Mutations that disrupt the proper morphogenesis and localization of a sperm-specific organelle in Caenorhabditis elegans. Dev. Biol. 134: 307-316.

Hill, DP, DC Shakes, S. Ward, and S. Strome. (1989). A sperm-supplied product essential for initiation of normal embryogenesis in Caenorhabditis elegans is encoded by the paternal-effect embryonic-lethal gene, spe-11. Dev. Biol. 136(1): 154-166.

L’Hernault, SW, DC Shakes, and S. Ward. (1988). Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode, C. elegans. Genetics 120(2): 435-452.